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KMID : 0624620090420100691
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BMB Reports
2009 Volume.42 No. 10 p.691 ~ p.696
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Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells
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Shen Yan-Nan
Kim Yoon-Mo
Yun Cheol-Heui
Moon Yang-Soo
Kim Sang-Hoon
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Abstract
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The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.
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KEYWORD
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Adipocyte, E2F1, Pref-1, Promoter, Transcriptional activation
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